中华医学会系列杂志
Chinese Journal of Clinicians ISSN 1674-0785 CN 11-9147/R 联系我们网站地图帮助中心
期刊导读
期刊存档

您的位置:首页>> 文章摘要

肿瘤自分泌相关因子TGFβRⅡ对MKN-45人胃癌调控作用的体外及体内研究

叶敏 矫建鹏 张璇 武峰 裴蓓 魏品康 岳小强 孙大志

200003 上海,第二军医大学长征医院中医科

孙大志,Email: sunda-zhi@163.com

国家自然科学基金(81273910)

摘 要:目的 从肿瘤自分泌相关因子TGFβRⅡ探讨消痰散结方对MKN-45人胃癌调控作用。方法 采用体外细胞培养和体内动物实验的方法,Weston blot法检测不同组别TGFβRⅡ的蛋白含量;real-time PCR法检测不同组别TGFβRⅡ的基因表达。结果 体外实验中TGFβRⅡ的蛋白含量分别为空白对照组0.342±0.032、空白药物血清组0.359±0.043、消痰散结方血清组0.825±0.143、替加氟血清组0.969±0.077,四组间比较差异有统计学意义(P<0.01),消痰散结方组与空白对照组、空白药物血清组比较差异均有统计学意义(P<0.01);real-time PCR法检测体外实验中不同组别TGFβRⅡ的基因表达:空白药物血清组6.17±0.77、消痰散结方血清组6.64±0.97、替加氟血清组9.13±2.12,虽组间比较差异无统计学意义(P>0.05),但替加氟组与消痰散结方组均有上调其表达的趋势,替加氟的上调趋势更明显。体内实验中各组TGFβRⅡ的蛋白含量分别为空白对照组0.597±0.04、生理盐水组0.692±0.091、消痰散结方组0.639±0.099、替加氟化疗组0.465±0.064,四组间表达差异有统计学意义(P=0.035),进一步两两比较,替加氟组与生理盐水比较差异有统计学意义(P=0.042)。表明替加氟组不能上调体内实验瘤中TGFβRⅡ的蛋白表达,消痰散结方组与空白对照组比较虽然差异未见统计学意义,但有上调趋势。体内实验各组TGFβRⅡ的基因表达分别为空白模型组1.56±0.39、消痰散结方组3.30±1.17、替加氟化疗组6.44±3.50,不同组别TGFβRⅡ的基因表达差异有统计学意义(P<0.01),替加氟组表达高于模型组及消痰散结方组(P<0.05),消痰散结方组与模型组虽然表达差异无统计学意义,但有明显的上调趋势。结论 消痰散结方对TGFβRⅡ蛋白和基因表达均有不同程度的上调作用,表明消痰散结方的治疗胃癌的作用可能是通过上调胃癌自分泌因子TGFβRⅡ发挥作用。

关键词:胃肿瘤; 自分泌因子TGFβRⅡ; 消痰散结方

Regulation effect of tumor autocrine related factor TGF beta RⅡ on the human gastric cancer MKN-45 cell and solid tumor

Ye Min, Jiao Jianpeng, Zhang Xuan, Wu Feng, Pei Bei, Wei Pinkang, Yue Xiaoqiang, Sun Dazhi.

Department of Traditional Chinese Medicine, Changzheng Hospital, the Second Military Medical University, Shanghai 200003, China

Sun Dazhi, Email: sunda-zhi@163.com[DOI:10.3877/cma.j.issn.1674-0785.2017.04.014

Abstract:Objective To investigate the regulation effect of Xiaotansanjie Recipe on gastric cancer MKN-45 based on tumor autocrine related factor TGF beta RⅡ. Methods Used the method in vitro cell culture and in vivo animal experiment, Weston blot method was used to detect the protein content of TGF beta RⅡ in different groups and detected gene expression of TGF beta RⅡ in different groups by real-time PCR. Results The protein content of in vitro TGF beta RⅡ were control group 0.342±0.032, blank drug serum group 0.359±0.043, Xiaotansanjie Recipe group 0.825±0.143, tegafur group 0.969±0.077, respectively. There was significant difference between the four groups (P<0.01). There was significant difference (P<0.01) between the comparison of Xiaotansanjie Recipe group and blank control group and Xiaotansanjie Recipe group and blank drug serum group. Detected TGF beta RⅡ gene expression by the method of real-time PCR in vitro: blank drug serum group 6.17±0.77, Xiaotansanjie group 6.64±0.97, tegafur group 9.13±2.12 respectively. There was no significant difference between the four groups (P>0.05), but both of tegafur group and Xiaotansanjie group had the trend of increasing TGF beta RⅡ expression. The trend of the increased expression of tegafur serum group was more obvious. In vivo experiment, the protein content of TGF beta RⅡ in each group were control group 0.597±0.04, saline group 0.692±0.091, Xiaotansanjie Recipe group 0.639±0.099, tegafur chemotherapy group 0.465±0.064, respectively. There was significant difference among the four groups (P=0.035). There was significant difference between the treatment group and the saline group (P=0.042). The results showed that the tegafur group could not raise the expression of TGF beta RⅡ protein in vivo. There was no significant difference between Xiaotansanjie group and control group, but the upward trend was presented. In vivo experiment the expression levels of TGF beta R Ⅱ genes: blank model group 1.56±0.39, Xiaotansanjie group 3.30±1.17, tegafur chemotherapy group 6.44±3.50, respectively. There was significant difference among the three groups in the expression of TGF beta RⅡ (P<0.01). Tegafur group was higher than that in the model group and Xiaotansanjie group (P<0.05). Although the expression difference was not statistically significant, there was obvious upward trend between Xiaotansanjie Recipe group and model group. Conclusion Xiaotansanjie Recipe may upregulate TGF beta RⅡ protein and gene expression in different degrees, which indicate that the treatment effect of Xiaotansanjie Recipe on gastric carcinoma may play a role through upregulation of autocrine factor TGF beta RⅡ.

Key words:Stomach neoplasms; Autocrine factor TGFβRⅡ; Xiaotansanjie recipe

评论   收藏 全文阅读: FullText |

文献标引:叶敏 矫建鹏 张璇 武峰 裴蓓 魏品康 岳小强 孙大志.肿瘤自分泌相关因子TGFβRⅡ对MKN-45人胃癌调控作用的体外及体内研究[J/CD].中华临床医师杂志:电子版,2017,11(4):596.

参考文献:
  [1] Shi Y, Massague J. Mechanisms of TGF-beta signaling from cell membrane to the nucleus[J]. Cell, 2003, 113(6): 685-700.

  [2] Sun DZ, Jiao JP, Ju DW, et al. Tumor interstitial fluid and gastric cancer metastasis: an experimental study to verify the hypothesis of "tumor-phlegm microenvironment"[J]. Chin J Integr Med, 2012, 18(5): 350-358.

  [3] Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015[J]. CA Cancer J Clin, 2016, 66(2): 115-132.

  [4] Wrana JL. TGF-beta receptors and signalling mechanisms[J]. Miner Electrolyte Metab, 1998, 24(2/3): 120-130.

  [5] 贾英民, 武密山, 李恩. Smads蛋白介导的TGF-β超家族信号转导的研究进展[J]. 疑难病杂志, 2008, 7: 4.

  [6] Busch S, Acar A, Magnusson Y, et al. TGF-beta receptor type-2 expression in cancer-associated fibroblasts regulates breast cancer cell growth and survival and is a prognostic marker in pre-menopausal breast cancer[J]. Oncogene, 2015, 34(1): 27-38.

  [7] Xu Q, Zhang XM, Duan KZ, et al. Peripheral TGF-beta1 signaling is a critical event in bone cancer-induced hyperalgesia in rodents[J]. J Neurosci, 2013, 33(49): 19099-19111.

  [8] 武艳, 袁晓环, 张羽飞, 等. 原发性肝癌患者外周血中TGF-β1 mRNA表达水平的检测及其临床意义[J]. 中国生化药物杂志, 2010, 31: 3.

  [9] Piestrzeniewicz-Ulanska D, Brys M, Semczuk A, et al. TGF-beta signaling is disrupted in endometrioid-type endometrial carcinomas [J]. Gynecol Oncol, 2004; 95(1): 173-180.

  [10] Yashiro M, Hirakawa K, Boland CR. Mutations in TGFbeta-RⅡ and BAX mediate tumor progression in the later stages of colorectal cancer with microsatellite instability[J]. BMC Cancer, 2010, 10: 303.

  [11] Ogino S, Kawasaki T, Ogawa A, et al. TGFBR2 mutation is correlated with CpG island methylator phenotype in microsatellite instability-high colorectal cancer[J]. Hum Pathol, 2007, 38(4): 614-620.

  [12] Chen GT, Zhu ZG, Yin HR, et al. The relationship between frameshift mutations of transforming growth factor-beta type Ⅱ receptor, insulin growth factor Ⅱ receptor, bcl-2 associated X protein, E2F4 and microsatellite instability in gastric carcinoma[J]. Zhonghua Wai Ke Za Zhi, 2006, 44(5): 344-348.

  [13] 刘昕燕, 高岩, 魏明洁, 等. 口腔鳞癌及癌前病变中TGFβRⅡ的表达[J]. 现代口腔医学杂志, 2000, 14(2): 219-210.

  [14] 刘正新, 陈宝雯. PCNA、EGFR、TGFβRⅠ和TGFβRⅡ在胃癌组织中表达的临床意义[J]. 中国肿瘤临床, 2005, 32(7): 378-381.

  [15] 李相勇, 魏品康. 金龙蛇口服液治疗晚期胃癌的疗效观察[J]. 湖北中医杂志, 2001, 23(11): 3-5.

  [16] 秦志丰, 魏品康, 李相勇. 金龙蛇口服液合参麦注射液对中晚期胃癌患者肿瘤标志物和免疫功能的影响[J]. 中医杂志, 2001, 42(2): 605-606.

基础论著

活性氧激活内质网诱导软骨细胞凋亡的研究

刘超1 夏长所1 李晓飞1 金丹凤2 吕成昱1. .中华临床医师杂志:电子版 2017;11(4):591.

摘要 FullText 评论 收藏

肿瘤自分泌相关因子TGFβRⅡ对MKN-45人胃癌调控作用的体外及体内研究

叶敏 矫建鹏 张璇 武峰 裴蓓 魏品康 岳小强 孙大志. .中华临床医师杂志:电子版 2017;11(4):596.

摘要 FullText 评论 收藏

抗炎治疗促进脑梗死后脑内神经发生和功能恢复的实验研究

吴晓巍1 孟庆敏2 张晓芳3 毛今明3 李常新1 李新果3. .中华临床医师杂志:电子版 2017;11(4):602.

摘要 FullText 评论 收藏

凉膈散对内毒素致急性肺损伤大鼠高迁移率族蛋白1影响

周勇1 赵玮2. .中华临床医师杂志:电子版 2017;11(4):607.

摘要 FullText 评论 收藏

血管内皮生长因子在哮喘小鼠的表达及细辛脑的干预作用

宫晓丹1,2 丁雁启3 宫冰2 黄传君4 张才擎2. .中华临床医师杂志:电子版 2017;11(4):612.

摘要 FullText 评论 收藏

H型高血压大鼠模型的建立及评价

曹艳丽1,2 徐瑞2. .中华临床医师杂志:电子版 2017;11(4):616.

摘要 FullText 评论 收藏