中华临床医师杂志(电子版) 2017年2月,11卷4期

临床论著

骨折延迟愈合相关miRNAs检测

陈磊 刘黎军 张胜利 杨金星 黄俊锋 尤微 韩云

518035 广东省,深圳市第二人民医院创伤骨科
刘黎军,Email: 13923405112@163.com

摘要:目的 检测骨折延迟愈合患者血浆中差异表达的miRNAs,研究参与骨折愈合过程中的miRNAs。方法 2014年10月至2016年6月在深圳市第二人民医院骨科入院治疗的骨折患者。骨折患者按照骨折愈合情况分为延迟愈合组和正常愈合组,用Agilent miRNA芯片检测延迟愈合和正常愈合患者血浆miRNAs差异表达谱,在34例骨折延迟愈合患者和32例骨折正常愈合患者血浆中用Real-time RT-PCR对芯片检测差异表达的miRNAs进行验证,组间数据差异比较用t检验。结果 与骨折正常愈合相比,有28个miRNAs在骨折延迟愈合患者中异常表达(差异2倍以上),其中13个基因表达水平增高,15个基因表达水平下降。Real-time RT-PCR 验证结果显示,hsa-miR- 145-3p在延迟愈合组比正常愈合组表达水平增高8.46倍(P=0.001)。结论 hsa-miR-145-3p可能参与了骨折延迟愈合的发生过程。有可能成为骨折延迟愈合的分子标记和治疗靶基因。

关键词:骨折愈合; 骨折延迟愈合; 微RNAs; hsa-miR-145-3p

深圳市科技计划(JCYJ20150330102720177);深圳市第二人民医院临床一对一项目

Screening differentially expressed miRNAs in delayed union of fractures

Chen Lei, Liu Lijun, Zhang Shengli, Yang Jinxing, Huang Junfeng, You Wei, Han Yun.

Department of Orthopaedics, Shenzhen Second People's Hospital, Shenzhen 518035, China
Liu Lijun, Email: 13923405112@163.com

Abstract:Objective To dectect the differential expression of plasma miRNAs in delayed union of fractures group and study the miRNAs involved in fracture healing process. Methods Selected fracture patients for the treatment from October 2014 to June 2016 in orthopedics department in the Shenzhen Second People's Hospital. Fracture patients were divided into delayed union of fractures group and non-delayed union of fractures group according to the conditions of fracture healing. Plasma miRNA expression of delayed union of fractures was analyzed using Agilent miRNA microarray. Aberrant expression of miRNAs was further verified by Real-time RT-PCR in 34 delayed union of fractures and 32 non-delayed union of fractures patients. The difference between groups was evaluated by t-test. Results 28 aberrant miRNAs were identified by miRNA microarray (at least>2 folds) in delayed union of fractures group, including high expression of 13 miRNAs and low expression of 15 miRNAs. Meantime, Real-time RT-PCR determined that the expression of hsa-miR-145-3p was increased about 8.46 folds (P=0.001) in delayed union of fractures group compared to non-delayed union of fractures group. Conclusion hsa- miR-145-3p may be involved in the process of delayed union of fractures and may be molecular markers and therapeutic targets for delayed healing of fractures.

Keywords:Fracture healing; Delayed union of fractures; MicroRNAs; hsa-miR-145-3p

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(编辑:马超 收稿日期:2016-11-03)