中华临床医师杂志(电子版) 2017年2月,11卷4期

临床论著

水通道蛋白3表达及催乳素浓度与羊水量异常相关性

徐静 许红雁 范舒舒 黄伟

512026 广东韶关,汕头大学医学院附属粤北人民医院妇产科1,病理科2
许红雁,Email: gdsgxhy@21cn.com

摘要:目的 研究水通道蛋白3(AQP3)表达及催乳素浓度与羊水量异常的相关性,为临床防治羊水量异常提供依据。方法 选取汕头大学医学院附属粤北人民医院2014年5月至2016年4月收治的74例行选择性剖宫产健康单胎孕妇作为研究对象,入院后均接受超声检查,并根据超声检查羊水指数(AFI)进行分组。26例AFI≤50 mm的羊水过少组孕妇为观察组A,36例AFI>50 mm且<250 mm的羊水量正常组孕妇为对照组(B组),12例AFI≥250 mm的羊水过多组孕妇为观察组C;采集三组产妇外周血、胎盘标本,外周血采用电化学发光法检测催乳素含量,胎盘组织采用免疫组织化学法检测羊膜上皮细胞、胎盘滋养细胞、绒毛膜滋养细胞的AQP3表达水平。观察并比较三组孕妇外周血催乳素浓度及羊膜上皮细胞AQP3表达水平。结果 羊膜上皮细胞、胎盘滋养细胞、绒毛膜滋养细胞上均有AQP3表达。羊膜上皮细胞的AQP3表达水平由低到高依次为:观察组A<对照组<观察组C,差异有统计学意义(P<0.05);三组孕妇母血内催乳素浓度差异无统计学意义。催乳素浓度与羊水量异常无明显相关性。结论 AQP3在羊膜上皮细胞的表达可能对羊水膜内吸收的调节具有重要影响,可能是羊水量增多时的代偿改变;而母体外周血催乳素浓度对羊水量异常无显著性影响。

关键词:水通道蛋白3; 催乳素; 羊水量; 相关性研究

Correlation study on aquaporins 3 expression plus prolactin concentration and amniotic fluid volume abnormality

Xu Jing1, Xu Hongyan1, Fan Shushu1, Huang Wei2.

1Department of Obstetrics and Gynecology, 2Department of Pathology, Yuebei People's Hospital of Shantou University Medical College, Shaoguan 512026, China
Xu Hongyan, Email: gdsgxhy@21cn.com

Abstract:Objective To study the correlation of aquaporins 3 (AQP3) expression plus prolactin concentration and amniotic fluid volume abnormality, and to provide reference to clinical treatment of amniotic fluid volume abnormality. Methods 74 healthy singleton pregnant women admitted into our hospital from May 2014 to April 2016 were selected as research object. They received B ultrasound in the hospital and were divided into groups by amniotic fluid index (AFI). 26 cases of oligohydramnios (AFI≤50 mm) were A observation group, 36 cases of normal amniotic fluid volume (50 mmcontrol group>A observation group (P<0.05); There was no difference in the prolactin concentration in maternal blood among the three groups. The prolactin concentration had no significant correlation with amniotic fluid volume abnormality. Conclusion AQP3 expression in amniotic epithelial cells possibly has important effect on regulation of amniotic fluid membrane absorption, which is possibly compensatory change when amniotic fluid volume increases; while prolactin concentration in maternal peripheral blood has no significant effect on amniotic fluid volume abnormality.

Keywords:Aquaporin-3; Prolactin; Amniotic fluid volume; Correlation study

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(编辑:戚红丹 收稿日期:2016-12-26)