中华临床医师杂志(电子版) 2017年2月,11卷4期

基础论著

H型高血压大鼠模型的建立及评价

曹艳丽1,2 徐瑞2

250014 济南,山东中医药大学1;250014 济南,山东大学附属千佛山医院心内二科2
徐瑞,Email: xuruicn@hotmail.com

摘要:目的 构建并评价H型高血压大鼠模型的方法。方法 选取自发性高血压大鼠(SHR)16只和同源正常血压大鼠(WKY)8只,适应性喂养一周。SHR大鼠随机分为普通饮水组和2%蛋氨酸饮水组,WKY大鼠为普通饮水组,每周记录每组大鼠进食量、饮水量、体质量、体质量增加量、收缩压(SBP)、舒张压(DBP)变化。1个月后测定血浆中同型半胱氨酸(Hcy)浓度。结果 24只大鼠全部进入结果分析。(1)各组大鼠每日平均进食量、饮水量比较,SHR 2%蛋氨酸饮水组均低于SHR双蒸水组和WKY双蒸水组(P<0.01);而SHR双蒸水组与WKY双蒸水组差异无统计学意义(P>0.05);(2)各组大鼠体质量随着时间的增加而增加,体质量增加量随着时间的增加而减少;各组大鼠平均每周体质量增加量,SHR 2%蛋氨酸饮水组与SHR双蒸水组和WKY双蒸水组差异无统计学意义(P>0.05);SHR双蒸水组与WKY双蒸水组差异无统计学意义(P>0.05)。(3)各组大鼠每周SBP比较发现,SHR 2%蛋氨酸饮水组DBP在各个时间点均明显高于SHR双蒸水组和WKY双蒸水组(P<0.01),SHR双蒸水组在各个时间点上均高于WKY双蒸水组(P<0.05);(4)各组大鼠每周DBP比较发现,SHR 2%蛋氨酸饮水组DBP在0、2、3、4周均明显高于SHR双蒸水组和WKY双蒸水组(P<0.01),在1周时,SHR 2%蛋氨酸饮水组与SHR双蒸水组DBP均高于WKY双蒸水组(P<0.01),SHR 2%蛋氨酸饮水组DBP与SHR双蒸水组差异无统计学意义(P>0.05);(5)SHR 2%蛋氨酸饮水组Hcy水平高于SHR双蒸水组和WKY双蒸水组(P<0.01);而SHR双蒸水组与WKY双蒸水组差异无统计学意义(P>0.05)。结论 通过蛋氨酸饮水与SHR结合可以构建H型高血压大鼠模型。

关键词:蛋氨酸; 同型半胱氨酸; 高血压; 模型,动物

To establish and evaluate a rat model of hypertension with elevated plasma homocysteine

Cao Yanli1,2, Xu Rui2.

Shandong University of Traditional Chinese Medicine, Jinan 250014, China; 2Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Jinan 250014, China

Abstract:Objective To establish and evaluate a rat model of hypertension with elevated plasma homocysteine. Methods Sixteen SPF spontaneously hypertension rats (SHR) male of 9 weeks old and eight SPF Wistar Kyoto rats of 9 weeks old were adaptively bred for one-week. SHR were randomly divided into double distilled water drinking group and 2% methionine water drinking group with 8 animals in each group. All WKY rats were given double distilled water. Food intakes, water intakes, body mass, body weight increment, SBP and DBP were detected each week. Plasma homocysteine was detected after 1 month. Results Twenty-four rats were all included in the final analysis. (1) As for average daily food intake and average daily water intake, SHR 2% methionine water drinking group was significantly lower than SHR double distilled water drinking group and WKY double distilled water drinking group (P<0.01). There was no significant difference in the average daily feed intake and average daily water intake between SHR double distilled water drinking group and WKY double distilled water drinking group (P>0.05); (2) The body weight of the rats increased with the increase of time, but the increment of rat's body weight increased decreased as the time goes on. In addition, no significant difference was found in the increment of rat’s body weight between three groups (P>0.05). (3) Compared with SHR double distilled water drinking group and WKY double distilled water drinking group, SHR 2% methionine water drinking group was higher obviously in systolic blood pressure of every week. (4) In the time of 0 week, 2 weeks, 3 weeks, 4 weeks, diastolic blood pressure was significantly higher in 2% methionine water drinking group than in SHR double distilled water drinking group and WKY double distilled water drinking group (P<0.01). At the same time, SHR double distilled water drinking group was higher than WKY double distilled water drinking group (P<0.01). There is no significant difference in DBP between SHR double distilled water drinking group and 2% methionine water drinking group in 1 week (P>0.05). However, SHR 2% methionine water drinking group and SHR double distilled water drinking group was higher than WKY double distilled water drinking group (P<0.01); (5) In respect to the level of plasma homocysteine, SHR 2% methionine water drinking group was significantly higher than SHR double distilled water drinking group and WKY double distilled water drinking group (P<0.01). There was no significant difference in the level of plasma homocysteine between SHR double distilled water drinking group and WKY double distilled water drinking group (P>0.05). Conclusion Methionine water drinking and SHR can induce the rat model of hypertension with elevated plasma homocysteine.

Keywords:Methionine; Homocysteine; Hypertension; Models, animal

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(编辑:戚红丹 收稿日期:2016-11-04)